Kuidas kasutada an optiline mikroskoop
1. Toiming sammud ja ettevaatusabinõud
(1) püstine mikroskoop
1. Hoia the peegel käsivars koos oma parem käsi ja peegel alus koos sinu vasak käsi to keep the peegel keha püsti. The desktop should be clean and stable, and you should choose a place near a window or with sufficient light. The single tube is general placed on the left side, 3 to 4 cm away from the edge of the table.
2. Puhas ja kontroll kas mikroskoop on vigane ja puhas. Meie mehaanilised osad peegel korpus saab pühkida a puhas pehme riie. Ekraan peaks olema pühkida lääts lääts puhastamine paber. Kui on liim või mustus, puhas see koos a väike kogus ksüleen.
3. Tõstke the peegel tünn to a kaugus 1 to 2 cm from the stage, and align the low-suurendus peegel koos valgus auk. reguleeri ava ja reflektor, use lame peegel millal valgus on tugev, kasutamine nõgus peegel millal valgus on nõrk, helkur tahe pööre koos mõlema käega. If a mikroskoop koos a valgus allikas on kasutatakse, see samm saab olla ära jätta, aga a nupp for reguleerimine the heledus on nõutav.
4. Install the specimen. Put the slide on the stage, and pay attention that the side with the cover glass must face up. Secure the slide with spring clips, and turn the nupp of the stage mover so that the material to be observed is aligned with the center of the aperture.
5. When focusing, first turn the coarse focus knob to lower the lens barrel slowly, and observe carefully from the side until the objective lens is close to the slide specimen, then observe from the eyepiece with the left eye, and turn the coarse focus knob with the left hand to raise the lens barrel. Stop until the specimen image is clearly seen, and then use the fine focus knob to adjust it back to clear. Operation Note: Do not adjust the focus directly under a high-magnification lens; when the lens barrel is lowered, observe the distance between the lens barrel and the specimen from the side; know the critical value of the object distance. If you use a binocular microscope, if there is a difference in the diopter of the observer's eyes, you can use the diopter adjustment circle to adjust it. In addition, the binoculars can be relatively translated to adapt to the distance between the operator's eyes.
6. Vaatlus Kui sa kasutad a monokulaarne mikroskoop, avatud sinu silmad loomulikult, vaatle isend koos vasak silm, rekord ja joonista koos parem silm, ja at sama aeg reguleeri fookus koos vasak käsi to make the objekt pilt selge ja liikumine isend väli vaade. Parem käsi kirjed ja joonistused. Ajal mikroskoopiline uuring, isend isend peaks olema liigutatud in a kindel suund kuni kuni kogu isend on vaadeldav, so as mitte to miss or kordus. reguleerimine valgus intensiivsus: üldine, valgus for värvitud isendid peaks olema tugev, ja valgus värvitu või värvitu isendid peaks olema nõrk; valgus jaoks vähese võimsusega vaatlus peaks olema nõrk, ja valgus suure võimsusega vaatlus peaks olema tugev. in lisamine to reguleerimine helkur või valgus allikas, reguleerimine of the the iris of the iris ava on ka väga oluline.
(1) Vaatlus koos a vähese suurendusega objektiiv Millal vaatlemine suvaline isend, a vähese võimsusega objektiiv peab olema kasutatud esimene, sest see on a suur väli of vaade, ja see on lihtne leida the target and determine the part to be observed.
(2) Kõrge suurendus vaatlus Millal pööramine alates madal suurendus to kõrge suurendus, sina ainult vajadus veidi reguleerimine the peen fookus nupp to make the object image clear. Do not use the coarse focus nupp when using a high suurendus lens, as this can easy crush the coverslip and damage the lens. when objective lens converter, do not use your fingers to directly push the objective lens. See saab lihtsalt põhjustada optiline telg of eesmärk lääts to olla kõrvalekaldumine, ja kruvi keerme of the konverter tahe kahjustub tõttu kuni ebaühtlane jõud, ja lõpuks konverter tahe lammutatakse.
(3) Observation of the oil lens First use the low power lens and the high power lens to move the inspected object to the center of the field of view, and then change the oil lens for observation. Before oil lens observation, the brightness of the microscope should be adjusted to the brightest, and the aperture should be fully opened. When using an oil lens, first drop a drop of cedar oil (lens oil) on the cover glass, then lower the lens barrel and observe carefully from the side until the oil lens is immersed in the cedar oil and is close to the slide specimen, then observe with the eyepiece, and use the fine adjustment The focus knob lifts the lens barrel until the focal length of the specimen is seen clearly and then stops and adjusted clearly. Cedar oil should be added in an appropriate amount. After using the oil lens, be sure to wipe off the cedar oil with a lens cleaning paper dipped in xylene, and then wipe off the excess xylene with a dry lens cleaning paper.
7. Finish the operation. After observing, remove the sample, reverse the converter, make the lens V-shaped to the sides, and the mirror should be erected. Lower the lens barrel, wipe it clean, and put on the lens cover. If you are using a microscope with a light source, you need to adjust the brightness knob to adjust the brightness to the darkest, and then turn off the power button to prevent the instantaneous excessive current from burning out the light source when you turn it on next time.
(2) Inverted Microscope The main difference between an inverted microscope and an upright microscope is that the objective lens is located below the stage, which is conducive to performing some real-time operations on the sample above during observation. The operation process of an inverted microscope is basically similar to that of a binocular upright microscope. The following points should be noted: the hinged binocular eyepieces can be adjusted to a comfortable position during observation. Splashes of tissue culture fluid or water onto the stage, objective, or microscope frame may damage the equipment. If spilled, immediately unplug the power cord from the wall outlet and wipe up the spilled liquid or water. Be sure to turn the light intensity knob gently and do not try to turn the knob past the end position. After use, be sure to adjust the intensity of the light to the minimum before turning off the power. After use, the three-hole converter should be rotated so that the objective lens is placed on the lower side of the stage to prevent dust from settling.
(3) Solid microscope is also called stereo microscope or dissecting microscope. The operation steps are basically similar to those of a binocular upright microscope: when using a dissecting mirror, you need to use both hands to move and keep steady. If the mirror box needs to be moved, the mirror box should be locked, and the key of the mirror box must be removed at the same time. If there is a dust cover on the mirror tube, it should be removed and replaced with the eyepiece and eyecup. Place the sample on a glass slide or in a wax dish and place it on a loading plate for observation. Tighten the screws, raise the mirror body to a certain height first, and then lock the mirror body. The eyepiece tube can be turned to fit the eye distance before observation. The difference in binocular vision can be adjusted by the visual circle. When focusing, do not turn the lifting screw too fast or forcibly, so as not to damage the gear. For magnified observation, turn the magnification dial until the required magnification. The larger the object image, the darker the light, so it is necessary to adjust the light source and select a good background object. After use, first take away the things on the loading tray, and tighten the elastic screws to put down the mirror body and lock it. Remove the eyepiece and replace the dust cover. Put the elements back together, being careful not to interchange with other mirrors. Wipe the mirror body clean with a cloth, put it into the mirror box, and lock the mirror box.
